RNA
Part:BBa_K4506055:Design
Designed by: Juan Manuel Dominguez Larrieta Group: iGEM22_Tec-Monterrey (2022-10-13)
sRNA for CAM-R silencing (AI generated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 104
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The complete sRNA sequence (hybridization region + Hfq binding domain) was optimized for having better structural and folding characteristics according to AI detailed by our team
Source
The sRNA-mRNA pairing sequence (24 bp) was designed by our team. The HFQ binding domain was obtained from the iGEM parts registry (BBa_K1137009)